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Research Groups

Lennart Brodin

Lennart Brodin, Ph.D.

Research Focus

We are studying the molecular basis of neurotransmitter release with focus on the mechanisms of synaptic vesicle recycling. One current main interest concerns the functions of synaptic proteins with lipid-binding domains, such as BAR and ENTH domains. We are also very interested in the mechanisms underlying presynaptic actin regulation, and the link between actin dynamics and vesicle traffic. Another project deals with the mechanisms that control trafficking of SNARE proteins during synaptic vesicle cycling. Our main model is the lamprey giant reticulospinal synapse. In this synapse the presynaptic axon is very large (diameter 100 m) and the active zone are separated from each other, which gives unique possibilities to dissect mechanisms acting at distinct steps of the synaptic vesicle cycle. A combination of molecular, electrophysiological, and light and electron microscopic imaging techniques are used.

Selected Publications

Brodin L., Lw P., Shupliakov O.

Sequential steps in clathrin-mediated synaptic vesicle endocytosis.

Current Opinions in Neurobiology 10: 312-320, 2000

Gad H., Ringstad N., Lw P., Kjaerulff O., Gustafsson J., Wenk M., Di Paolo G., Nemoto Y., Crum J., Ellisman M., De Camilli P., Shupliakov O., Brodin L.

Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin.

Neuron 27, 301-312, 2000

Shupliakov O., Bloom O., Gustafsson J., Kjaerulff O., Lw P., Pieribone V., Greengard P., Brodin L.

Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton.

Proceedings of the National Academy of Sciences U.S.A. 99: 14476-14481, 2002

Andersson, F., Jakobsson, J., Lw, P., Shupliakov, O., Brodin, L.

Perturbation of syndapin/PACSIN impairs synaptic vesicle endocytosis evoked by intense stimulation.

Journal of Neuroscience 28: 3925-3933, 2008

Jakobsson, J., Gad, H., Andersson, F., Lw, P., Shupliakov, O., Brodin, L.

Role of epsin in synaptic vesicle endocytosis.

Proceedings of the National Academy of Sciences U.S.A. 2008 (in press).

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