Realtime PCR Primers

Abbreviations and color codes
 

A B C D E F G H I J K L M N O P Q R S T U V X Y Z

A
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
ATF-4 920-938:19 agaagaagagggctgagca 1001-1019:19 tctttggcgagagaatctg NM_024403 100 MAL/EED
Akt1/PKB-a 627-640:21 gcttactgagaaccgtgtcct 889-905:17 atgtgcccgtccttgtc D30040 279 MAL/EED
Akt2/PKB-b 1548-1566:19 ctgccacccttcaaacctc 1712-1730:19 tgctcactctcggatgctg D30041 183 MAL/EED
Akt3/PKB-g 984-1006:23 tggcaccagaggtattagaagat 1168-1187:20 agagccctgaaagcaatgac D49836 204 MAL/EED
AChR-D cagccgtctacagtgggatg ctgccagtcgaaagggaagta
AChR-E attgaagagcttagcctgta tacacctgcaaaatcgtcct
AChR-G gatgcaatggtgcgactatcgc gcctccgggtcaatgaagatcc

B
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
Beta Actin 607-626:20 (2401-2420) acagctgagagggaaatcgt 875-895:21 (2757-2777) gcactgtgttggcatagaggt V01217 289 MAL/EED
Beta Actin exon 2232-2252:21 tcgtaccactggcattgtgat 2443-2464:22 cgaagtctagggcaacatagca V01217 233 MAL/EED
Beta Actin intron 1860-1878:19 ctcgatcgcctttctgact 1996-2015:20 gacaggggctccatttagac V01217 156 MAL/EED

C
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
Carbonic anhydrase III 304-321:18 cgccaattccatcttcac 478-495:18 gcctttctcccgtcctat AF037072 192 MAL/EED
CARP 462-481:20 agtataaacggaccgctctc 643-659:17 tgtctcgggcactgatt L81174 198 MAL/EED
CARP (bakup) 30-50:21 aggagctggtaacgggcaaaa 164-184:21 ttcttcccccaggttcacact L81174 155 MAL/EED
Caveolin-1 129-146:18 cgcgcacaccaaggaaat 212-230:19 ccttctggttccgcaatca NM_031556 102 Doyle
Caveolin-3 447-471:25 cctgcattaagagctacctgattga 545-565:21 tcgcagcaccaccttaatgtt U31968 119 Doyle
Catalase 1588-1609:22 cccagaagcctaagaatgcaat 1638-1658:21 agcttttcccttggcagctat M11670 71 MAL
Cathepsin B 278-296:19 gggttcagcgaggacataa 410-428:19 tgcagattcggtcagacat NM_022597 151 MAL
Cathepsin D 605-624:20 cgtattcatcgcagccaagt 769-788:20 gccgccaagcattagttctc NM_134334 184 MAL
Cathepsin E 575-594:20 atcgcagtccagcacctaca 747-766:20 cccagaatcccgtcaaactc NM_012938 192 MAL
Cathepsin K 734-754:21 acgagaaagccctgaagagag 844-864:21 cacattatcacggtcgcagtt NM_031560 131 MAL
Cathepsin L 360-379:20 tatcgccaccagaagcacaa 480-499:20 aaagcccagcaagaaccaca NM_013156 140 MAL
Cathepsin S 681-701:21 agctgccacgtgttcaaggta 869-889:21 caagagtcccatagccaacca NM_017320 209 MAL
CREB 850-868:19 tccccagcacttcctacac 973-992:20 agcactgccactctgttctc X14788 143 MAL/EED
Cyclophilin 314-335:22 agcatacaggtcctggcatctt 366-388:23 tgcagataaaaaactgggaacca M19533 75 MAL

D
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

E
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
ERK1 687-704:18 gggctacaccaaatccat 872-891:20 ggttttagagggcagagact X65198 205 MAL/EED
ERK2 233-251:19 cgcgctacactaatctctc 408-424:17 tgatgccgatgatgttc M64300 192 MAL/EED
ERK5/BMK1/MAPK7 572-591:20 ccagtcacttgtgccaccta 792-809:18 aaagccaacaccgtagcc XM_213323 238 MAL/EED

F
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
Follistatin 354-371:18 tgccccagactgttccaa 438-421:18 gaggagcgcacattcgtt NM_012561 85 MAL/EED
Follistatin 354-371:18 tgccccagactgttccaa 446-465:20 ttccggctgctctttacatc NM_012561 112 MAL/EED
Foxo1/FKHR 754-770:17 tacggccaatccagcat 877-896:20 tggggaggagagtcagaagt AF247812 143 MAL/EED
Foxo3/FKHRL1/FKHR2 55-74:20 cggctcactttgtcccagat 198-217:20 tcttgccagtcccttcgttc XM_215421 163 MAL/EED
Foxo4/AFX 1433-1454:22 gtgctcgcatctcctactgaag 1506-1525:20 catgtcgcactccaggttct AB032770 93 MAL/EED
FoxoK1 (MNF) 953-972:20 tctacgcccacatcaccaaa  1093-1112 gcagggtctattcgccaaaa  NM_010812 (mo) 160 MAL

G
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
g-GCS 128-149:22 gcacatctaccacgcagtcaag 233-252:20 cgccgccattcagtaacaac J05181 125 MAL/EED
GAPDH 818-840:23 acctgccaagtatgatgacatca 872-891:20 agcccaggatgccctttagt X02231 74 MAL
GAPDH 763-787:25 tccgtgttcctacccccaatgtatc 1017-1040:24 caccaccctgttgctgtagccata X02231 255 EBB
GLUT4 1502-1521:20 tccttctatttgccgtcctc 1672-1690:19 ttgcccctcagtcattctc X14771 189 MAL/EED
GDNF 391-408:18 aaatcgggggtgcgtctt 562-581:20 atgcctggcctaccttgtca  L15305 191 MAL/EED
GDNF 359-377:19 agagggaaaggtcgcagag 432-451:20 gtagcccaaacccaagtcag L15305 93 MAL/EED
GFRalpha1 1815-1834:20 tttcacccaactggaactct 1888-1908:21 ggtttggaattagccctgtag U59486 94 MAL/EED
Glutaredoxin 1 100-119:20 cccctattgcagaaagactc 269-286:18 atcactgcatccgcctat NM_022278 187 MAL/EED
Glutaredoxin 2 230-248:19 tcgttttgggggaagtcag 320-340:21 ggccattgaacagtaagagca XM_213890 111 MAL/EED
GPx1 198-217:20 ccgggactacaccgaaatga 271-291:21 ctgatgtccgaactgattgca X12367 94 MAL
GPx4 275-295:21 aatgaggcaaaaccgacgtaa 327-347:21 cgtaaaccacactcggcgtat U37427 73 MAL
GSR 299-316:18 tgagccgcctgaacaaca 351-369:19 tgcgtagccgtggatgact U73174 71 MAL
GSS 1109-1127:19 tgctgcccctagccacttt 1167-1188:22 cgtgtaccatttcctccccata L38615 80 MAL

H
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
histone deacetylase 2 (Hdac2) 349-369:21 ggtcgtaggaatgtcgctgat 482-502:21 atttggctcctttggtgtctg XM_215410 154 MAL/EED

I
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
IGF-I sign - B-pep 59-77 / 810-828:19 tcttctacctggcactctg 110-126 / 861-877:17 gcaaagggtctctggtc D00698M1548 68 MAL/EED
IGF-I signal spec 108-126: gtcgtcttcacatctcttc 139-155: gtaaaggtgagcaagca M15480
IGF-I pre - signal 80-97 gcgcacctccaataaaga 117-138 gagtgccaggtagaagagatgt M15480
IGF-I pre spec 38-60:23 ctctgacctgctgtgtaaacgac 69-88:23 gaggtgcgctcattttggta M15480
IGF-I mature 273-290:18 ggtggacgctcttcagtt 332-350:19 tacatctccagcctcctca
IGFbp5  1196-1217:22 cccaactgtgaccgcaaaggat 1328-1347:20 tcgaaggcgtggcactgaaa NM_012817 152 MAL/EED
I-kappa B alpha 491-509:19 tgcaggccaccaactacaa 567-586:20 cagcacccaaagtcaccaag XM_234230 96 MAL/EED
I-kappa B beta 982-1003:22 gcccgaggataaggatgataag 1101-1120:20 ggaaggaggtggctggtttt NM_030867 139 MAL/EED
IRS-1 3812-3832:22 acctaagtcccaaccataacca 4002-4023:22 tctcagccacacattctcaaag X58375 212 MAL/EED

J
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

K
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

L
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
LMP2 113-130:18 tcgtggtgggctctgatt 333-353:21 tcgcggtacttgtaggagatg D10757 241 MAL/EED

M
Molecule Sense primer pos:lgth Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
Methyltransferase (MT)
(0-6-methylG-DNA MT)		 325-342:18 cttgcctgctctccatca 527-544:18 accgtcactgcgaatcac  NM_012861 220 MAL/EED
atrogin-1/MAFbx 219-243:25 ccatcaggagaagtggatctatgtt 274-293:20 gcttcccccaaagtgcagta AY059628 75 Dehoux et al
Murf1 297-315:19 tgtctggaggtcgtttccg 336-355:20 atgccggtccatgatcactt NM_080903 59 Latres et al
MRF4 (myf6) 629-649:21 gggcctggtgataactgctaa 684-703:20 gaaaggcgctgaagactgct M27151 75 MAL/EED
Myf5 378-396:19 tgccatccgctacattgag 518-537:20 gttctttcgggaccagacag X56182 160 MAL/EED
MyoD(myf3) 3715-3735:21 gacgccgcctactacagtgag 4277-4298:22 gtaaatcggattgggctttgag M84176 269(584) EED
Myogenin(myf4) 237-260:24 gggcgtgtaaggtgtgtaagagga 703-725:23 cagcctggcagacaatctcagtt M24393 489 EED
Myogenin 694-712:19 accatgcccaactgagatt 862-880:19 tgggcagggtgttagtctt M24393 187 MAL/EED
Myostatin agagagaggcgaatgtggaa tcactgctgtcatccctctg 226 Dasarathy S

N
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
NFK-B-p50 (p105) 15-34:20 tccccctgagaaagaaacac 117-134:18 tcctgaaaccccacatcc L26267 120 MAL/EED
NFK-B-p65 (REL-A) 1056-1075:20 tgtgcctacccgaaactcaa 1144-1163:20 aacagcatgggggaaaactc M61909 108 MAL/EED

O
Molecule Sense primer pos:lgth Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

P
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
PA28alpha 185-204:20 agccaacctgagcaatctga 300-319:20 tcgtcttcgtcccctttctt D45249 135 MAL
PA28beta 79-96:18 gagtggagaagcccgtaa 151-167:17 tccgtggcaagaaagtg D45250 89 MAL
PAC1 265-282:18 ggcgttcctttcatcgtt 424-443:20 agcagctacactggcagaga XM_213645 179 MAL
PAC2 133-152:20 cccgacctaaagaacttcac 266-283:18 ggcaccaggcaatcagta XM_214550 134 MAL
PCNA cacagcatgctccaatatggc acttggaatcccagaacagg 100 Dasarathy S
p21 cccgtggacagtgagcagtt agcagggccgaggaggta 233 Dasarathy S
p21 not tested nor orderd CTGCCCAAGCTCTACCTTCC CAGGTCCACATGGTCTTCCT 123 Schonherr E
p70S6K 806-825:20 acagtcacgcacacattttg 1043-1062:20 gcagcatttcttttcagcag M57428 257 MAL/EED
p90RSK 774-793:20 ggccattgaccacgaaaaga 879-901:23 caaacatcaacaccccataggac D83013 128 MAL/EED
PI3K, PIK3c1a (p110a) Ras binding reg 444-462:19 gccaaagcacatctacaac 489-507:19 gactatcacccaaatcacc XM_227039 64 MAL/EED
PI3K, PIK3c1b (p110b) 2847-2866:20 tggcattaaaagggaacgag 2968-2986:19 gccgccgtaaaatcaggta NM_053481 140 MAL/EED
PI3K, PIK3r (p85-specific) 362-381:20 agtttgcccctcctgatgtt 558-575:18 tcggcgagatagcgtttg D64045 214 MAL/EED
PI3K, PIK3r (reg sub-u iso unspec) 1891-1908:18 cgaaacaaagccgagacc 1984-2003:20 ttgacttcgccatctaccac D64045 113 MAL/EED
POMP 326-345:20 ggttcagcgtcttccgtttc 431-450:20 gctcgcccatcagttcactt XM_213700 125 MAL
POMP (alt. primer) 187-206:20 ttttgcccagtcaccctctt 431-450:20 gctcgcccatcagttcactt XM_213700 264 MAL
Proteasome subunit LMP2 113-130:18 tcgtggtgggctctgatt 333-353:21 tcgcggtacttgtaggagatg D10757 241 MAL
Proteasome subunit LMP7 563-582:20 ctctcgggacagatgttctc 738-755:18 cccaaccgtctttcttca NM_080767 193 MAL
Proteasome subunit RCX 528-548:21 agacagtgaggggaacaggat 706-725:20 cgcacatggtagaggttgac D45247 198 MAL
Proteasome subunit Y 191- 210:20 acaagctgacccctatccac 383-401:19 cctgcgatgatgattcctg D10754 211 MAL
Proteasome subunit Z 358-377:20 cagagttgtcacagcgaatc 628-646:19 tccagaccccaagtcatta AF285103 289 MAL
Proteasome subunit, beta Type 1 gca tca ggg agg aga ctg tt taa aac aaa cgt gcc aca gt BC018351 Kane JK
Proteasome subunit, beta Type 2 ccc cca cag cag cag cta at agc cag cca gga gga ggt tc AF060090 Kane JK
Proteasome subunit, beta Type 3 gcg tca ttg tcc aca tca tt gcc aga gca cag gtt agt cc BC014783 Kane JK
Proteasome C3 cattcagcccatctggtaaacttgt ctgtacaccaaaccgatgtgctt 196 Dasarathy S
Proteasome C5 cctgtgcagatgcgtttctcg aaagccactgcagccaattactgtt 159 Dasarathy S
Proteasome C9 agctgagtaaagcggcgctgatctg tcccaaacaagtgcctgcgtgtc 155 Dasarathy S
Proteasome ATPase p45/SUG aac cag ctg gat ggc ttt ga gca ggg cag agt cca gga ta AB000491 Kane JK
Proteasome 26S subunit, ATPase 3 gac ctg gac tcg cag agg aa aac cca atc act ggc agg aa NM_031595 Kane JK
Proteasome subunit 26S, non-ATPase 4 cag gac cca gag ttc ctt ca att gca ttt cga atg gct tc NM_031331 Kane JK
Proteasome 26S subunit, non-ATPase 13 gtt tgg cac cgt ttg gaa ga gca tgg gtc ctg cac aaa at NM_011875 Kane JK

Q
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

R
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
Ret 629-648:20 atagtctgcccttccgttgt 790-808:19 ccgagtcgtcctcatcata X67812 180 MAL/EED
ribosomal protein S18 369-386:18 tggagcggctgaagaaaa 410-427:18 acgaaggccccaaaagtg X57529 59 MAL/EED
ribosomal protein S20 182-199:18 gaagtcgctggaaaaggt 340-357:18 gtcgcttgtggattctca X51537 176 MAL/EED
rRNA, 18S rRNA 961-981:21 cgccgctagaggtgaaattct 1011-1033:23 tgaaaacattcttggcaaatgct M11188 (V01270) 73  MAL
H-RAS 228-247:19 gggcttcctctgtgtatttg 427-445:19 gggtcttggctgatgtttc M13011 218 MAL/EED
K-RAS 223-240:18 ggggagggctttctttgt 430-450:21 ctgtcttgtcttcgctgaggt U09793 228 MAL/EED
N-RAS 304-323:20 cgggtgaaagactctgatga 534-551:18 agccccatacagccttga X68394 248 MAL/EED

S
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
ShcA pan 1601-1621:21 ctgaaggtgtggttcggacaa 1674-1694:21 gcagagatgatgggcaagtga (mo)U46956.2 94 MAL/EED
ShcA p66 265-283:19 gaagatggggaagggagtg 421-440:20 cctcgtgtgggcttattgac (mo)U46956.2 176 MAL/EED
ShcA (p52, p66) 447-464:18 gcatcccaacgacaaagt 539-558:20 ctgagtccgggtattgaagt (mo)U46956.2 112 MAL/EED
ShcB (Sck) 92-111:20 ccggggtctcttacatcgtt  157-176:20 tgtgtccgggtgttgaaatc AB001452 85 MAL
ShcC (N-SHC) mo 246-265:20 gagaaagccgccgagtaaga 400-419:20 ccccctgaggcaaaagagat U46854 (mo) 174 MAL
ShcC (N-SHC p52, p66) 1624-1643:20 caaagctgcgtctgtggagt 1712-1731 ctgctcatctcgccttggta  AB001453 108 MAL
SOD1 103-122:20 aaggccgtgtgcgtgctgaa 250-269:20 gtacagccttgtgtattgtc NM_017050 167 Kono
SOD2 511-530:20 gctggcttggcttcaataag 595-614:20 aatccccagcagtggaataa NM_017051 104 Pang
SOD3 501-518:18 atgagttcggggacctga 816-835:20 cttcttgcgctcctttgtct NM_012880 335 MAL

T
Molecule Sense primer pos:lgth Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
Tripeptidylpeptidase II (TPP II) 3209-3228:20 gaaaaggagcggatgaaaag 3285-3304:20 ggcaatgtaaacagcgagag U50194 96 MAL/EED
Tubulin alpha V01227 330 MAL/EED
Tubulin alpha (over exon 2&3)  268-290:23 gtagacctggaacccacagttat 387-406:20 tctccttgccaatggtgtag V01227 139(296) MAL/EED
Tubulin alpha exon 4 484-501:18 agctttggtgggggaact 570-589:20 gggctgggtaaatggagaac V01227 106 MAL/EED
Tubulin alpha intron C 592-611:20 agtgggagggtgtcttggta 712-730:19 gtcagcctgtgggagaaaa J00798 139 MAL/EED
Thioredoxin tccaatgtggtgttccttga atagaactggaaggtcggca AF311055 93 RTPrimer DB

U
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref
Ubiquitin C tcttcgtgaagaccctgacc caggtgcagggttgactctt 200
14-kDa E2 gtgcaccatctgaaaacaa atcggttctgcaggatgtct 221
ubiquitin B-like (rat) tga gga ctg cag cca aca cc ggt gtc act ggg ctc gac ct D16554 Kane JK
Ubiquitin-conjugating enzyme 5 ggc cag aca gtg gac caa ga cac ccc cac cct ttc aaa at BC003781 Kane JK
Ubiquitin-like protein 1 (SUMO-1) cca gga ggc aaa acc ttc aac cac tgc tat cct gtc caa tga c NM_009460 Kane JK
Ubiquitin-like protein 2 (SUMO-2) ggg aca gga tgg ttc tgt gg caa acc ctg ccg ttc aca at NM_133594 Kane JK
Ubiquitin-conjugating enzyme E2I gac ttg cgc agg aga gga aa tcg tgc cat cag ggt tct tt NM_013050 Kane JK

V
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

X
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

Y
Molecule Sense primer pos:lgth Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref

Z
Molecule Sense primer pos:lgth  Sense primer sequence (5'-3') Antisense primer pos:lgth Antisense primer sequence (5'-3') Accession no Prod
bp		 Ref